Plasmid

Part:BBa_K4630115:Design

Designed by: Zhejun Qin   Group: iGEM23_WHU-China   (2023-10-10)


pCDF-Duet1-T7-stgRNA-cassette 3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1519
    Illegal XbaI site found at 3753
    Illegal SpeI site found at 1511
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1519
    Illegal NheI site found at 2953
    Illegal SpeI site found at 1511
    Illegal NotI site found at 1536
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1519
    Illegal BglII site found at 1692
    Illegal XhoI site found at 1741
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1519
    Illegal XbaI site found at 3753
    Illegal SpeI site found at 1511
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1519
    Illegal XbaI site found at 3753
    Illegal SpeI site found at 1511
    Illegal NgoMIV site found at 1711
    Illegal NgoMIV site found at 2206
    Illegal AgeI site found at 1953
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is worth noting that the casstte should remain intact on the cloning, and Lac operator is essential for better control of IPTG.


Source

Complete synthesis and Golden Gate Assembly.

References